| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhao, Jayden W (10)
|
AMRXA |
37.87 |
38.38 |
0.51 |
| 2 |
Chen, Nathan Y (10)
|
MDCAA |
40.14 |
39.57 |
-0.57 |
| 3 |
Basilio, Maxwell D (10)
|
PVNCAP |
44.08 |
43.37 |
-0.71 |
| 4 |
Chen, Luohan K (9)
|
PVDRAG |
43.40 |
43.68 |
0.28 |
| 5 |
Mancari, Ezra J (9)
|
PVPM |
44.50 |
43.72 |
-0.78 |
| 6 |
Cawley, Austin V (10)
|
MDNAAC |
46.38 |
44.20 |
-2.18 |
| 7 |
Vu, Grant H (10)
|
MDCAA |
44.71 |
44.64 |
-0.07 |
| 8 |
Manning, Greyson J (10)
|
PVPM |
45.79 |
44.72 |
-1.07 |
| 9 |
Wu, Leo (10)
|
PVDRAG |
43.32 |
44.83 |
1.51 |
| 10 |
Sherry, Bennett E (9)
|
PVPM |
48.41 |
45.28 |
-3.13 |
| 11 |
Powell-Switaj, Benton C (10)
|
PVPM |
49.43 |
46.81 |
-2.62 |
| 12 |
Hopp, Carter C (10)
|
MDCAA |
54.62 |
53.82 |
-0.80 |
| 13 |
Ferssizidis, Haralabos (10)
|
PVPM |
1:10.25 |
57.09 |
-13.16 |
| 14 |
Anisko, Matthew S (10)
|
MDCAA |
1:10.38 |
1:00.74 |
-9.64 |
|
Tao, Bryan B (10)
|
MDCAA |
44.65 |
NS |
- |